Dentin constitutes the major component of dental mineralized tissue, and is produced by cranial neural crest cell-derived odontoblasts are highly differentiated post-mitotic cells secreting the organic matrix of dentin throughout the life of the human beings. The aim of this study was to identify the expression pattern of four major noncollagenous protein of dentin, osteocalin(OC), osteonectin(ON), alkaline phosphatase(ALP), and dentin-specific protein, dentin sialophosphoprotein(DSPP).
Explants from human dental pulp were cultured in DMEM supplemented with 10% fetal bovine serum. Total RNA was isolated from cultured human pulp cells and converted using reverse transcriptase and the resulting cDNA was amplified using specific primers.
The results as following:
1. OC and ON was expressed in the cultured dental pulp cells. In contrast, ALP expression was not detected in cultured human dental pulp cells.
2. Dentin-specific protein, DSPP, was also expressed in cultured human dental pulp cells.
These results suggested that odontoblast could differentiated from the human dental pulp cells and the early differentiated odontoblasts expressed OC, ON and dentin-specific protein. DSPP except for ALP which related to the dentin mineralization process.
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